Reay-to-Use PCR Master Mix
Realtime PCR Master Mix(with dNTPs，lyophilized) is a ready-to-use, lyophilized master mix for high-efficiency realtime PCR amplification which can be used for rapid detection of DNA samples.. The mix include double- blocked hot-start Super HP Taq DNA polymerase, MgCl2 and dNTPs. Simply reconstitute the master mix by adding PCR-grade water along with your template, Taqman probes and primers to a total volume of 20 µl.
Since the PCR products generated by lyophilized PCR Master Mix contain 3'-A overhangs, they can be directly cloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY133-01 and KY133-02 are packaged in individual with different specifications of 8-well strip. Each pack contains one strip with 8 reactions(8T), and 6 × 8-well strip for a kit. It can be used individually or all at once, the rest of the 8- well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY133-03 is a premix frozen dried preparation of 24 reactions (that is, 24T) per tube, with a total of two tubes. Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time.
KY133-04 is a premix frozen dried preparation of 500 reactions (that is, 500T) per tube, and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time
Realtime PCR Master Mix(with dNTPs，lyophilized) is a ready-to-use, lyophilized master mix for high-efficiency realtime PCR amplification which can be used for rapid detection of DNA samples.. The mix include double- blocked hot-start Super HP Taq DNA polymerase, MgCl2 and dNTPs. Simply reconstitute the master mix by adding PCR-grade water along with your template, Taqman probes and primers to a total volume of 20 µl.
Since the PCR products generated by lyophilized PCR Master Mix contain 3'-A overhangs, they can be directly cloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY133-01 and KY133-02 are packaged in individual with different specifications of 8-well strip. Each pack contains one strip with 8 reactions(8T), and 6 × 8-well strip for a kit. It can be used individually or all at once, the rest of the 8- well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY133-03 is a premix frozen dried preparation of 24 reactions (that is, 24T) per tube, with a total of two tubes. Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time.
KY133-04 is a premix frozen dried preparation of 500 reactions (that is, 500T) per tube, and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time
Realtime PCR Master Mix(with dNTPs，lyophilized) is a ready-to-use, lyophilized master mix for high-efficiency realtime PCR amplification which can be used for rapid detection of DNA samples.. The mix include double- blocked hot-start Super HP Taq DNA polymerase, MgCl2 and dNTPs. Simply reconstitute the master mix by adding PCR-grade water along with your template, Taqman probes and primers to a total volume of 20 µl.
Since the PCR products generated by lyophilized PCR Master Mix contain 3'-A overhangs, they can be directly cloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY133-01 and KY133-02 are packaged in individual with different specifications of 8-well strip. Each pack contains one strip with 8 reactions(8T), and 6 × 8-well strip for a kit. It can be used individually or all at once, the rest of the 8- well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY133-03 is a premix frozen dried preparation of 24 reactions (that is, 24T) per tube, with a total of two tubes. Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time.
KY133-04 is a premix frozen dried preparation of 500 reactions (that is, 500T) per tube, and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×Realtime PCR Master Mix premixel liquid (that is 10μL/T), and place ice bath for later use. The frozen -drying preparation after re-dissolving should be used up at one time