Ready-to-Use 1st Strand cDNA Synthesis Master Mix
PRODUCT INTRODUCTION
Ready-to-Use 1st Strand cDNA Synthesis Master Mix is a premixed lyophilized reagent for PCR efficient amplification. It is designed for first-strand synthesis of full-length cDNA. It can synthesize full-length cDNA (up to 2kb) for downstream experiments such as cloning and highly homogeneous cDNA for quantitative PCR. It includes efficient M-MLV (H-) Reverse Transcriptase, RNase inhibitor, MgCl2, dNTPs and PCR buffer system. M-MLV (H-) Reverse Transcriptase has the following activities: heat and RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity;RNase H activity. RT-Buffer-Mix includes optimized buffer system and dNTP suitable for subsequent PCR, qPCR and PCR cloning. This kit does not include single-component reverse transcription primer Oligo (dT), Random hexamers and gene specific primers (GSP). Users can flexibly select reverse transcription primers for subsequent experiments as needed.It includes the new and efficient M-MLV (H-) Reverse Transcriptase so it has reliable reverse transcription efficiency: for RNA templates above 100ng, it has stable and reliable reverse transcription performance. It includes optimized PCR buffer for better specificity.
CAT no. KY188-01 and KY188-02 are lyophilized reagents in 0.1mL and 0.2mL PCR 8-tube strips, respectively, and both are sealed in aluminum foil bags. Each aluminum foil bag contains a PCR 8-tube strip, and each tube contains a single reaction lyophilized Ready-to-Use 1st Strand cDNA Synthesis Master Mix, which is sealed and stored under dry conditions. Each kit contains 6 sealed aluminum foil bags, a total of 48 tubes of individually packaged reagents. Single tube or multiple tubes can be used according to the needs of the experiment, and the remaining reagents and desiccant are transferred to the aluminum foil ziplock bag provided in the kit, sealed and stored at -25°C~8°C to avoid moisture. The reaction system of each tube of Ready-to-Use 1st Strand cDNA Synthesis Master Mix
lyophilized powder is 20μL.
CAT no. KY188-03 is a premixed lyophilized reagent with 24 reactions per tube (24T), which is stored dry in an aluminum foil bag. Before use, take out a tube of lyophilized reagent and add 250μL of PCR-grade water to reconstitute and vortex for 5-10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagent is recommended to be used up at one time.
CAT no. KY188-04 is a premixed lyophilized reagent with 500 reactions per tube (500T), which is stored dry in an aluminum foil bag. Take it out before use, add 5000μL of PCR-grade water to reconstitute the lyophilized powder and vortex for 5~10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix premix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagents are recommended to be used up at one time.
APPLICATION
This kit can be widely used in reverse transcription reactions of animal, plant and microbial RNA.
(1) First-strand cDNA synthesis for conventional PCR, quantitative PCR.
(2) Synthesize cDNA for cloning and expression.
(3) Construction of full-length cDNA library.
(4) Primer extension to analyze RNA.
PRODUCT INTRODUCTION
Ready-to-Use 1st Strand cDNA Synthesis Master Mix is a premixed lyophilized reagent for PCR efficient amplification. It is designed for first-strand synthesis of full-length cDNA. It can synthesize full-length cDNA (up to 2kb) for downstream experiments such as cloning and highly homogeneous cDNA for quantitative PCR. It includes efficient M-MLV (H-) Reverse Transcriptase, RNase inhibitor, MgCl2, dNTPs and PCR buffer system. M-MLV (H-) Reverse Transcriptase has the following activities: heat and RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity;RNase H activity. RT-Buffer-Mix includes optimized buffer system and dNTP suitable for subsequent PCR, qPCR and PCR cloning. This kit does not include single-component reverse transcription primer Oligo (dT), Random hexamers and gene specific primers (GSP). Users can flexibly select reverse transcription primers for subsequent experiments as needed.It includes the new and efficient M-MLV (H-) Reverse Transcriptase so it has reliable reverse transcription efficiency: for RNA templates above 100ng, it has stable and reliable reverse transcription performance. It includes optimized PCR buffer for better specificity.
CAT no. KY188-01 and KY188-02 are lyophilized reagents in 0.1mL and 0.2mL PCR 8-tube strips, respectively, and both are sealed in aluminum foil bags. Each aluminum foil bag contains a PCR 8-tube strip, and each tube contains a single reaction lyophilized Ready-to-Use 1st Strand cDNA Synthesis Master Mix, which is sealed and stored under dry conditions. Each kit contains 6 sealed aluminum foil bags, a total of 48 tubes of individually packaged reagents. Single tube or multiple tubes can be used according to the needs of the experiment, and the remaining reagents and desiccant are transferred to the aluminum foil ziplock bag provided in the kit, sealed and stored at -25°C~8°C to avoid moisture. The reaction system of each tube of Ready-to-Use 1st Strand cDNA Synthesis Master Mix
lyophilized powder is 20μL.
CAT no. KY188-03 is a premixed lyophilized reagent with 24 reactions per tube (24T), which is stored dry in an aluminum foil bag. Before use, take out a tube of lyophilized reagent and add 250μL of PCR-grade water to reconstitute and vortex for 5-10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagent is recommended to be used up at one time.
CAT no. KY188-04 is a premixed lyophilized reagent with 500 reactions per tube (500T), which is stored dry in an aluminum foil bag. Take it out before use, add 5000μL of PCR-grade water to reconstitute the lyophilized powder and vortex for 5~10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix premix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagents are recommended to be used up at one time.
APPLICATION
This kit can be widely used in reverse transcription reactions of animal, plant and microbial RNA.
(1) First-strand cDNA synthesis for conventional PCR, quantitative PCR.
(2) Synthesize cDNA for cloning and expression.
(3) Construction of full-length cDNA library.
(4) Primer extension to analyze RNA.
PRODUCT INTRODUCTION
Ready-to-Use 1st Strand cDNA Synthesis Master Mix is a premixed lyophilized reagent for PCR efficient amplification. It is designed for first-strand synthesis of full-length cDNA. It can synthesize full-length cDNA (up to 2kb) for downstream experiments such as cloning and highly homogeneous cDNA for quantitative PCR. It includes efficient M-MLV (H-) Reverse Transcriptase, RNase inhibitor, MgCl2, dNTPs and PCR buffer system. M-MLV (H-) Reverse Transcriptase has the following activities: heat and RNA-dependent DNA polymerase activity; DNA-dependent DNA polymerase activity;RNase H activity. RT-Buffer-Mix includes optimized buffer system and dNTP suitable for subsequent PCR, qPCR and PCR cloning. This kit does not include single-component reverse transcription primer Oligo (dT), Random hexamers and gene specific primers (GSP). Users can flexibly select reverse transcription primers for subsequent experiments as needed.It includes the new and efficient M-MLV (H-) Reverse Transcriptase so it has reliable reverse transcription efficiency: for RNA templates above 100ng, it has stable and reliable reverse transcription performance. It includes optimized PCR buffer for better specificity.
CAT no. KY188-01 and KY188-02 are lyophilized reagents in 0.1mL and 0.2mL PCR 8-tube strips, respectively, and both are sealed in aluminum foil bags. Each aluminum foil bag contains a PCR 8-tube strip, and each tube contains a single reaction lyophilized Ready-to-Use 1st Strand cDNA Synthesis Master Mix, which is sealed and stored under dry conditions. Each kit contains 6 sealed aluminum foil bags, a total of 48 tubes of individually packaged reagents. Single tube or multiple tubes can be used according to the needs of the experiment, and the remaining reagents and desiccant are transferred to the aluminum foil ziplock bag provided in the kit, sealed and stored at -25°C~8°C to avoid moisture. The reaction system of each tube of Ready-to-Use 1st Strand cDNA Synthesis Master Mix
lyophilized powder is 20μL.
CAT no. KY188-03 is a premixed lyophilized reagent with 24 reactions per tube (24T), which is stored dry in an aluminum foil bag. Before use, take out a tube of lyophilized reagent and add 250μL of PCR-grade water to reconstitute and vortex for 5-10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagent is recommended to be used up at one time.
CAT no. KY188-04 is a premixed lyophilized reagent with 500 reactions per tube (500T), which is stored dry in an aluminum foil bag. Take it out before use, add 5000μL of PCR-grade water to reconstitute the lyophilized powder and vortex for 5~10 seconds to make 2×Ready-to-Use 1st Strand cDNA Synthesis Master Mix premix (10μL/T), and store in ice bath for later use. The reconstituted lyophilized reagents are recommended to be used up at one time.
APPLICATION
This kit can be widely used in reverse transcription reactions of animal, plant and microbial RNA.
(1) First-strand cDNA synthesis for conventional PCR, quantitative PCR.
(2) Synthesize cDNA for cloning and expression.
(3) Construction of full-length cDNA library.
(4) Primer extension to analyze RNA.