one-step RT-qPCR Master Mix
One-step RT-qPCR Master Mix (lyophilized ) is a ready-to-use, lyophilized master mix for high-efficiency RT-qPCR amplification which can be used for rapid detection of DNA or RNA samples.. The mix include double-blocked hot-start Super HP Taq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2 and dNTPs.Simply reconstitute the master mix by adding PCR-grade water along with your template ,Taqman probes and primersto a total volume of 20 µl.
Since the PCR products generated by lyophilized RT-qPCR Master Mix contain 3'-A overhangs, they can be directlycloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY132-01 and KY132-02 are packaged in individual with different specifications of 8-well strip. Each pack containsone strip with 8 reactions(8T), and 6 × 8-well strip for a kit(48T). It can be used individually or all at once, the rest ofthe 8-well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY132-03 is a lyophilized preparation which contains 24 reactions per tube (that is, 24T/tube), and 2 ×1 tube for a kit(48T). Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.
KY132-04 is a lyophilized preparation which contains 500 reactions per tube (that is, 500T/tube), and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.
One-step RT-qPCR Master Mix (lyophilized ) is a ready-to-use, lyophilized master mix for high-efficiency RT-qPCR amplification which can be used for rapid detection of DNA or RNA samples.. The mix include double-blocked hot-start Super HP Taq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2 and dNTPs.Simply reconstitute the master mix by adding PCR-grade water along with your template ,Taqman probes and primersto a total volume of 20 µl.
Since the PCR products generated by lyophilized RT-qPCR Master Mix contain 3'-A overhangs, they can be directlycloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY132-01 and KY132-02 are packaged in individual with different specifications of 8-well strip. Each pack containsone strip with 8 reactions(8T), and 6 × 8-well strip for a kit(48T). It can be used individually or all at once, the rest ofthe 8-well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY132-03 is a lyophilized preparation which contains 24 reactions per tube (that is, 24T/tube), and 2 ×1 tube for a kit(48T). Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.
KY132-04 is a lyophilized preparation which contains 500 reactions per tube (that is, 500T/tube), and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.
One-step RT-qPCR Master Mix (lyophilized ) is a ready-to-use, lyophilized master mix for high-efficiency RT-qPCR amplification which can be used for rapid detection of DNA or RNA samples.. The mix include double-blocked hot-start Super HP Taq DNA polymerase, M-MLV reverse transcriptase (RNaseH-), MgCl2 and dNTPs.Simply reconstitute the master mix by adding PCR-grade water along with your template ,Taqman probes and primersto a total volume of 20 µl.
Since the PCR products generated by lyophilized RT-qPCR Master Mix contain 3'-A overhangs, they can be directlycloned into T-vectors. The RT-qPCR products are suitable for sequencing.
KY132-01 and KY132-02 are packaged in individual with different specifications of 8-well strip. Each pack containsone strip with 8 reactions(8T), and 6 × 8-well strip for a kit(48T). It can be used individually or all at once, the rest ofthe 8-well strip should be kept sealed to avoid moisture. Each tube contains one reaction (20μL-tube).
KY132-03 is a lyophilized preparation which contains 24 reactions per tube (that is, 24T/tube), and 2 ×1 tube for a kit(48T). Add 250μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.
KY132-04 is a lyophilized preparation which contains 500 reactions per tube (that is, 500T/tube), and 1 tube for a kit(500T). Add 5000μL PCR-grade water per tube, mix the reaction solution by 8s vortex, make into 2×One step RT-qPCR Master Mix reaction solution (that is 10μL/T), and place ice bath for later use. The lyophilized preparation after re-dissolving should be used up at one time.